Resultado da pesquisa (2)
Termo utilizado na pesquisa Pinto A.A.
#1 - Serodiagnosis of Babesia equi in horses submitted to exercise stress, p.179-183
Abstract in English:
ABSTRACT.- Baldani C.D., Machado R.Z., Raso T.F. & Pinto A.A. 2007. Serodiagnosis of Babesia equi in horses submitted to exercise stress. Pesquisa Veterinária Brasileira27(4):179-183. Departamento de Patologia Veterinária, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14870-000, Brazil. E-mail: zacarias@fcav.unesp.br
A complement fixation test (CFT), performed in microtitre plates, based upon the use of crude antigenic preparation of Babesia equi was adapted for the detection of antibodies in serum of infected horses. The indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) were also used for the immunodiagnosis of B. equi. Serum samples from 15 apparently healthy horses, previously conditioned to a high-speed equine treadmill, were taken before and after exercise. All the samples analyzed were positive for B. equi infection. There were no significant differences (P<0.01) between these 3 tests, or the condition of rest or stress. The combined use of CFT and IFAT or ELISA should be recommended in order to enable veterinary services to more efficiently prevent introduction of infected horses into disease-free areas.
Abstract in Portuguese:
ABSTRACT.- Baldani C.D., Machado R.Z., Raso T.F. & Pinto A.A. 2007. Serodiagnosis of Babesia equi in horses submitted to exercise stress. Pesquisa Veterinária Brasileira27(4):179-183. Departamento de Patologia Veterinária, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14870-000, Brazil. E-mail: zacarias@fcav.unesp.br
A complement fixation test (CFT), performed in microtitre plates, based upon the use of crude antigenic preparation of Babesia equi was adapted for the detection of antibodies in serum of infected horses. The indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) were also used for the immunodiagnosis of B. equi. Serum samples from 15 apparently healthy horses, previously conditioned to a high-speed equine treadmill, were taken before and after exercise. All the samples analyzed were positive for B. equi infection. There were no significant differences (P<0.01) between these 3 tests, or the condition of rest or stress. The combined use of CFT and IFAT or ELISA should be recommended in order to enable veterinary services to more efficiently prevent introduction of infected horses into disease-free areas.
#2 - Comparative evaluation of different administration methods of a vaccine prepared with the LaSota strain of Newcastle disease virus
Abstract in English:
A comparative study of different application methods of a vaccine prepared with the LaSota strain, in primary vaccination against Newcastle disease, was· carried out in broiler-chicks from immune parental stock. Primary vaccination of seven day old birds was performed by aerosol, eye drop instillation and drinking water. The aerosol method induced the highest response of the hemagglutination inhiniting antibody, which was significant at the 5% level of probability whem compared to the eyedrop and drinking water methods. Whem vaccinated birds were challenged, it was found that the aerosol method provided better protection than the drinking water method, significant at the 5% level of probability. However, no significant diferences could be detected between the aerosol and the eyedrop methods. There was no correlalion between the hemagglutination inhibiting antibody titers found and the protection to challenge.
Abstract in Portuguese:
Um estudo comparativo entre diferentes métodos de administração de vacina preparada com a estirpe LaSota, em primovacinação contra a doença de Newcastle, foi realizado em pintos de corte, procedentes de matrizes imunizadas. Empregando-se primovacinação aos sete dias de idade das aves, pelas vias aerógenas, ocular e oral; o método aerosol induziu melhor resposta de anticorpos inibidores da hemaglutinação (HI), com significância ao nível de 5% de probabilidade, em relação aos métodos ocular e oral. No teste de proteção ao desafio, verificou-se a supremacia do método aerosol em relação ao oral, com significância ao nível de 5% de probabilidade, entretanto, sem diferenças significativas entre os métodos aerosol e ocular. Os títulos de anticorpos inibidores da hemaglutinação, em têrmos de valores médios não guardaram correspondência com os índices de proteção ao desafio.
